Reflections of yoga practice on the physical performance of obese students / Reflexos da prática de ioga no desempenho físico de estudantes obesos / Reflejos de la práctica del yoga en el rendimiento físico de estudiantes obesos

ABSTRACT Introduction: Yoga practice is an activity recently implemented in the content of school physical education to guide students to participate actively in sports activities. Its impacts on the body and psychological health of students are constantly evaluated. Objective: Study the effect of yoga practice on the heart rate and cardiopulmonary capacity of obese students. Methods: 40 obese students from a high school were selected as volunteers for the experiment, randomly divided into an experimental group and a control group, with no change in location or duration of intervention between the groups. The students in the control group participated in regular school activities during physical education classes, while the experimental group performed yoga training during the same schedule. The duration was eight weeks. Results: After the experiment, the heart rate and lung function indexes of the students in the experimental group showed a statistical increase, without significant weight changes, but body fat and visceral fat were reduced, with improvement in their body composition and muscle lines. Conclusion: Yoga practice reflected a positive effect on the physical performance of obese students, positively impacting on heart rate and cardiopulmonary resistance of the students. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: A prática de ioga é uma atividade recentemente implementada no conteúdo da educação física escolar com o objetivo de orientar os estudantes a participarem ativamente das atividades esportivas, e seus impactos sobre a saúde corporal e psicológica dos estudantes são constantemente avaliados. Objetivo: Estudar o efeito da prática de ioga sobre a frequência cardíaca e a capacidade cardiopulmonar dos estudantes obesos. Métodos: 40 estudantes obesos de uma escola secundária foram selecionados como voluntários para o experimento, divididos aleatoriamente em grupo experimental e grupo de controle, sem alteração de local ou duração de intervenção entre os grupos. Os alunos do grupo de controle participaram das atividades regulares da escola durante as aulas de educação física, enquanto os alunos do grupo experimental realizam treinamento de ioga durante o mesmo horário. A duração foi de 8 semanas. Resultados: Após o experimento, os índices de frequência cardíaca e função pulmonar dos alunos do grupo experimental mostraram um aumento estatístico, sem alterações significativas no peso, mas a gordura corporal e a gordura visceral foram reduzidas, com melhora de sua composição corporal e linhas musculares. Conclusão: A prática de yoga refletiu um efeito positivo sobre o desempenho físico dos estudantes obesos, repercutindo positivamente sobre a frequência cardíaca e a resistência cardiopulmonar dos estudantes. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: La práctica de yoga es una actividad recientemente implementada en el contenido de la educación física escolar con el objetivo de orientar a los alumnos a participar activamente de las actividades deportivas, y sus impactos sobre la salud corporal y psicológica de los alumnos son constantemente evaluados. Objetivo: Estudiar el efecto de la práctica de yoga sobre la frecuencia cardiaca y la capacidad cardiopulmonar de estudiantes obesos. Métodos: 40 estudiantes obesos de un instituto de secundaria fueron seleccionados como voluntarios para el experimento, divididos aleatoriamente en grupo experimental y grupo de control, sin cambios en el lugar ni en la duración de la intervención entre los grupos. Los alumnos del grupo de control participaron en las actividades escolares habituales durante las clases de educación física, mientras que los del grupo experimental realizaron el entrenamiento de yoga en el mismo horario. La duración fue de 8 semanas. Resultados: Tras el experimento, los índices de frecuencia cardiaca y de función pulmonar de los alumnos del grupo experimental mostraron un aumento estadístico, sin cambios significativos en el peso, pero se redujeron la grasa corporal y la grasa visceral, con una mejora de su composición corporal y de las líneas musculares. Conclusión: La práctica de yoga reflejó un efecto positivo en el rendimiento físico de los estudiantes obesos, incidiendo positivamente en la frecuencia cardiaca y la resistencia cardiopulmonar de los estudiantes. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Reliability of Morphological Measurement of Gastrocnemius Using Ultrasonography in Different Positions / 中国康复理论与实践

Objective:To investigate the reliability of the morphological measure of medial gastrocnemius by musculoskeletal ultrasound imaging on the standing and prone positions, and compare the differences in the structural parameters of the medial gastrocnemius in different positions. Methods:From December, 2017 to May, 2018, 30 healthy young subjects were measured the muscle thickness, fascicle length and pennation angle of medial gastrocnemius on the prone and standing position respectively by two testers with musculoskeletal ultrasound. One of the testers re-tested three days later. The intra-class correlation coefficient (ICC), measurement standard error (SEM) and minimum detectable change (MDC) of all muscle morphological parameters in different positions were calculated respectively, and the differences of the morphological measurement of the medial gastrocnemius between these two positions were compared. Results:In the prone position, the ICC of inter-rater was 0.932 to 0.943 and the ICC of intra-rater was 0.880 to 0.915. In the standing position, the ICC of inter-rater was 0.922 to 0.938, and the ICC of intra-rater was 0.839 to 0.925. There were significant differences in muscle thickness, fascicle length and pennation angle of medial gastrocnemius in standing position and prone position (P < 0.01). Inter-rater SEM of muscle thickness and fascicle length was less in standing position than in prone position, while intra-rater MDC of pennation angle was less in prone position. Conclusion:It is reliable to measure medial gastrocnemius in standing and prone positions with ultrasonography. Morphological measurement of gastrocnemius is more accurate in standing position, while the measurement in prone position is more sensitive.

Associations of triple-phase enhancement multi-slice CT scan with histological differentiation and lymphovascular invasion in gastric cancer / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To explore the associations of triple-phase enhancement multi-slice CT scan with histological differentiation and lymphovascular invasion in advanced gastric cancer.</p><p><b>METHODS</b>Patients with gastric cancer undergoing surgical resection between January 2006 and December 2009 were included, all of whom underwent routine and two-phase contrast enhancement multi-slice CT examinations of the upper abdomen before surgery. Patients with incomplete data were excluded. Postoperative specimens were used for evaluation of histological differentiation, microscopic lymphovascular invasion, and CD34 and D2-40 expression. Associations between contrast enhancement ratio (CER) of triple-phase multi-slice CT scan and differentiation as well as lymphovascular invasion were analyzed.</p><p><b>RESULTS</b>A total of 64 patients were included. There were significant associations between CER and tumor differentiation (P<0.05). Compared to those without microvascular invasion, CER of the arterial phase in gastric cancer with microvascular invasion was significantly higher (0.61±0.28 vs. 0.46±0.14, P<0.05), and that of the arterial-parenchymal phase was significantly lower(1.81±0.39 vs. 2.28±0.80, P<0.05). However, gastric cancers with lymphatic invasion were associated with significantly higher CER of the parenchymal phase than their counterparts(1.25±0.57 vs. 1.00±0.35, P<0.05).</p><p><b>CONCLUSION</b>CER of triple-phase multi-slice CT scan can reveal the differentiation of gastric cancer and is associated with microvascular and lymphatic invasion.</p>

Killing effect of cytotoxicty T lymphocyte primed by two different methods preparation of dendritic cells pulsed with antigen on Tca8113 cells in vitro / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the feasibility of dendritic cells (DCs) vaccine on the therapy of tongue carcinoma and find the better way of antigen load.</p><p><b>METHODS</b>The antigen peptides of Tca8113 cells were obtained by acid eluted technique and repetitive freeze thaw method. Separating T cell and inducing dendritic cells were obtained from human peripheral blood monocyte. Divided into three groups: Weak acid elution method antigen group, anti-freeze-thaw method antigen group, and the control group (without tumor antigen). T cells and UCs were mixed to culture by different effector-target ratio. Using MTT assay measured the quantities of absorbance and calculated stimulation index. Dendrtic cells pulsed with antigen were mixed with T cells by different effector-target ratio. MIT assay was used to measure the quantities of absorbance and calculate killing rate.</p><p><b>RESULTS</b>DCs vaccine was constructed successfully. DCs vaccine can induce T lymphocytes to kill Tca8113 cells and display the dose-effect relationship. There was significant difference among the three groups. The acid eluted and repetitive freeze thaw groups were better than the control group. The acid eluted group was better than repetitive freeze thaw group.</p><p><b>CONCLUSION</b>DCs vaccine can induce T lymphocytes to kill Tca8113 cells. The antigen peptides obtained by acid eluted technique is better than repetitive freeze thaw method in immunotherapy of tongue cancer.</p>

Multi-slice CT contrast-enhanced presentations of advanced gastric cancer: associations with histo-differentiation and expression of p53 and P-glycoprotein / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>This study aimed to investigate multi-slice CT contrast-enhanced presentation of gastric cancer and its correlation with histo-differentiation and p53 and P-glycoprotein (P-gp) expression.</p><p><b>METHODS</b>Sixty-six patients with gastric cancer in the present study underwent a multi-slice CT preoperative routine and dual-phase contrast-enhanced examination of the upper abdomen; postoperative specimens were used to determine histo-differentiation and the expression of p53 and P-gp. The correlation of multi-slice CT contrast-enhanced presentation with histo-differentiation and expression of p53 and P-gp was analyzed.</p><p><b>RESULTS</b>The dual-phase contrast-enhanced ratio (CER) was not correlated with the histo-differentiation of gastric cancer (P > 0.05). Positive expression of p53 and P-gp was significantly higher in the cases of layered or heterogeneous enhancement than in the cases of homogenous enhancement (P < 0.05). Positive expression of p53 was also correlated with the arterial phase CER, tumor size and lymph node metastasis (P < 0.05), but not with infiltration thickness of the gastric wall, nor was it correlated with the portal phase CER (P > 0.05). Positive expression of P-gp was only correlated with the portal phase CER (P = 0.005).</p><p><b>CONCLUSIONS</b>Differently enhanced pattern and CER of the arterial and portal phase in gastric cancer correlate with its different histo-differentiation and expression of p53 and P-gp respectively. In addition, tumor size and lymph node metastasis of gastric cancer relate to the expression of p53.</p>

Study of increased sensitivity on Tca8113 cell line to cisplatin by nm23-H1 in vitro / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the mechanism of sensitivity variation to cisplatin caused by nm23-H1.</p><p><b>METHODS</b>The samles was divided into two groups: Tca8113 group and Tca8113/nm23-H1 group. Using MTT and flow cytometer, the changes of cell mortality rate, apoptosis and mitochondrial membrane potential were detected. By VG PQ Excell, the changes of the intracellular platinum were detected.</p><p><b>RESULTS</b>In vitro the cell mortality rate and apoptosis were increased in Tca8113/nm23-H1 group, comparing with Tea8113 group. Mitochondrial membrane potential was decreased in Tca8113/nm23-H1 group. The intracellular platinum was increased significantly in Tca8113/nm23-H1 group. This effect could be inhibited by oubain which was an inhibitor of Na+/K+-ATP.</p><p><b>CONCLUSION</b>nm23-H1 can increase the sensitivity of cisplatin on Tca8113 cell line. The mitochondrial membrane potential was decreased by nm23-H1 so that intracellular platinum was increased and finally increased the apoptosis or necrosis.</p>

Effect on xenograft of nude mouse by combination therapy of nm23-H1 and protein-cisplatin / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the effect of protein-cisplatin and nm23-H1 therapy on the tumor of nude mouse.</p><p><b>METHODS</b>The 15 BALB/C female mice were divided into three groups, control group, protein-cisplatin group and protein-cisplatin+nm23-H1 plasmid group. Tca8113 were injected into the mice subcutaneously with the concentration of 3.1 x 10(6) cells/mL. After two weeks, the mixture of lipofectin and nm23-H1 was injected around xenograft of nude mouse. After three days, the protein-cisplatin was injected around xenograft. The weight of mouse, the volume and the weight of xenograft were measured.</p><p><b>RESULTS</b>The weight of mouse was lightest in control group. The volume and weight of the transplanted tumor were lightest in nm23-H1 +protein-cisplatin group.</p><p><b>CONCLUSION</b>The combination therapy of nm23-H1 and protein-cisplatin can effectively inhibites the growth of xenograft in nude mouse.</p>

The study on the regeneration of skeletal muscles after denervation / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the degeneration and regeneration of skeletal muscle after denervation.</p><p><b>METHODS</b>Denervation was carried out in gastrocnemius muscles in 30 adult BALB/C mice by cutting the sciatic nerve. The gastrocnemius muscles were removed at 1, 2, 4, 8, 12, 16 weeks after denervation, respectively. Specimens were processed for histological study and immunohistochemical technique.</p><p><b>RESULTS</b>Muscle fiber atrophy followed by degerneration and regeneration was observed in the early period of denervation. Fusion of the regenerated muscle cells with each other followed by degeneration of the cells and growth of fibro-connective tissue were observed in the later stage. The expression of myoglobin and actin decreased in 1-4 weeks after denervation. The postive expression of the proteins was observed in some 8 weeks' cells and in many degenerated 12-14 weeks' muscle cells.</p><p><b>CONCLUSION</b>Degeneration and regeneration may coexisted in the denervated muscles. The regenerated muscle cells can't fully develop due to the deficit of nerve regulation and degenerate again. The regenerated muscle cells will melt each other and can't develop to mature muscle fiber in the later stage.</p>